PCR-identification of Mycosphaerella species associated with leaf diseases of Eucalyptus
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منابع مشابه
PCR-identification of Mycosphaerella species associated with leaf diseases of Eucalyptus.
A PCR-based technique based on the ITS1-5.8s-ITS2 domain of the rRNA gene for identifying five species associated with Mycosphaerella leaf disease (MLD) of eucalypts was developed. Primer pairs MC2F and MC2R; ML1F and ML1R; MM1F and MM1R; MN1F and MN1R; and MP1F and MP1R amplified a product for DNA extracted from their single target species, those being M. cryptica, M. lateralis, M. marksii, M....
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Commercial Eucalyptus plantations provide an important source of hardwood for forestry industries, worldwide. Several species of Mycosphaerella are associated with a destructive Eucalyptus leaf disease known as Mycosphaerella Leaf Blotch (MLB). During 2000, a survey was undertaken in several commercial Eucalyptus growing areas of South Africa to determine the identity of the Mycosphaerella spp....
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متن کاملMycosphaerella species associated with Eucalyptus in south-western Australia: new species, new records and a key.
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متن کاملA specific primer PCR and RFLP assay for the rapid detection and differentiation in planta of some Mycosphaerella species associated with foliar diseases of Eucalyptus globulus.
It is difficult to accurately identify Mycosphaerella species associated with leaf diseases of Eucalyptus based on morphological characters, as there is considerable overlap between very similar species and subspecies, and isolation from the host is not easy. Thus, a PCR and RFLP assay based on the ITS region of nr DNA was developed for the rapid detection and differentiation of M. nubilosa, M....
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ژورنال
عنوان ژورنال: Mycological Research
سال: 2005
ISSN: 0953-7562
DOI: 10.1017/s0953756205003539